Prevalence of Blood Parasites (Trypanosoma spp.) in Snakehead Fish (Channa striata) Collected from a Fish Market in Aceh Besar Regency
Abstract
Snakehead fish (Channa striata) is recognized as a functional food due to its high protein and albumin content, which support tissue repair and wound healing. However, its susceptibility to haemoparasite infections, particularly Trypanosoma sp., may affect fish health and quality. This study aimed to determine the prevalence of haemoparasites in Channa striata and analyze associated factors such as sex, size, and body weight. A survey was conducted on 48 randomly selected fish obtained from a fish market in Aceh Besar Regency. Blood samples were collected and examined using Giemsa-stained thin blood smears under a microscope to detect Trypanosoma sp. The results showed that 6 out of 48 fish were infected, with a prevalence of 12.50%. Female fish showed a higher prevalence than males, and infection rates increased with fish size and body weight. In conclusion, haemoparasite infection is present in Channa striata and may influence its health and quality, highlighting the importance of monitoring and management to support its use as a functional food.
References
Alam, M. S., Projna, F., Zafrin, M. S., Das, R., & Khan, M. G. Q. (2022). Assessment of genetic diversity, detection of strain-specific single nucleotide polymorphisms and identification of the Bangladesh and Vietnam strain of Channa striata by PCR-RFLP analysis of the mitochondrial COI gene fragment. Aquaculture and Fisheries, 7(3), 287–295. https://doi.org/10.1016/j.aaf.2020.12.006
Alhayali, N. S., Mohammed, N. H., & Al-Lahaibi, B. Y. (2023). Detection of Blood Parasites in Fish. Journal of Pure and Applied Microbiology, 17(1), 421–426. https://doi.org/10.22207/JPAM.17.1.33
Amoutchi, A. I., Kersten, P., Vogt, A., Kohlmann, K., Kouamelan, E. P., & Mehner, T. (2023). Population genetics of the African snakehead fish Parachanna obscura along West Africa’s water networks: Implications for sustainable management and conservation. Ecology and Evolution, 13(1), 1–14. https://doi.org/10.1002/ece3.9724
Awal, Md. R., Attia, K. A., Khanom, M., Rahman, Md. A., Islam, Md. S., Mohammed, A. A., Hafez, Y. M., Mahmud, Y., & Bhadra, A. (2025). Evaluation of Biometric and Breeding Biology of the Striped Snakehead Channa striata in the Haor Region: Conservation and Effective Management. Aquaculture, Fish and Fisheries, 5(2), 1–7. https://doi.org/10.1002/aff2.70058
Benjakul, S., Sukkapat, P., Palamae, S., & Saetang, J. (2023). PCR-Based Method for Authentication of Meat and Processed Meat from Three Commercially Important Catfish Species. Food Analytical Methods, 16(5), 878–887. https://doi.org/10.1007/s12161-023-02475-8
Biswas, D., Choudhury, A., & Misra, K. K. (2001). Histopathology of Trypanosoma (Trypanozoon) evansi Infection in Bandicoot Rat. I. Visceral Organs. Experimental Parasitology, 99(3), 148–159. https://doi.org/10.1006/expr.2001.4664
Boonkusol, D., & Tongbai, W. (2016). Genetic variation of striped snakehead fish (Channa striata) in river basin of central thailand inferred from mtDNA COI gene sequences analysis. Journal of Biological Sciences, 16(1), 37–43. https://doi.org/10.3923/jbs.2016.37.43
Borst, P., Fase-Fowler, F., & Gibson, W. C. (1987). Kinetoplast DNA of Trypanosoma evansi. In Molecular and Biochemical Parasitology (Vol. 23, Number 1, pp. 31–38). https://doi.org/10.1016/0166-6851(87)90184-8
Chong, R. S. M. (2022). Trypanosomiasis. In S. B. Frederick, Kibenge, B. Bernardo, & S.-M. C. Roger (Eds.), Aquaculture Pathophysiology: Finfish Diseases: Volume I (1st ed., Vol. 1, pp. 601–609). Academic Press. https://doi.org/https://doi.org/10.1016/B978-0-12-812211-2.00048-2
Cioran, M. C., Pagu, I. B., Măgdici, E., Barbacariu, A. C., & Păsărin, B. (2017). Studies on strength of an optimal solution essential oil of clove mixed with alcohol required for anesthesia species Cyprinus carpio. Scientific Papers-Animal Science Series: Lucrări Ştiinţifice - Seria Zootehnie, 67, 110–113. https://www.uaiasi.ro/firaa/Pdf/Pdf_Vol_67/MC_Cioran.pdf
Corrêa, L. L., Oliveira, M. S. B., Tavares-Dias, M., & Ceccarelli, P. S. (2016). Infections of Hypostomus spp. By Trypanosoma spp. and leeches: A study of hematology and record of these hirudineans as potential vectors of these hemoflagellates. Revista Brasileira de Parasitologia Veterinaria, 25(3), 299–305. https://doi.org/10.1590/S1984-29612016049
Ferreira, M. L., & Avenant-Oldewage, A. (2013). Notes on the occurrence of Trypanosoma sp. (Kinetoplastida: Trypanosomatidae) in freshwater fishes from South Africa. Onderstepoort Journal of Veterinary Research, 80(1), 1–4. https://doi.org/10.4102/ojvr.v80i1.529
Fink, I. R., Ribeiro, C. M. S., Forlenza, M., Taverne-Thiele, A., Rombout, J. H. W. M., Savelkoul, H. F. J., & Wiegertjes, G. F. (2015). Immune-relevant thrombocytes of common carp undergo parasite-induced nitric oxide-mediated apoptosis. Developmental & Comparative Immunology, 50(2), 146–154. https://doi.org/10.1016/j.dci.2015.02.008
Hamad-Ali, S. J., & Ali, L. A. (2021). Piscicola geometra (Linnaeus, 1761) leech parasitizing two fish species hosts from Greater Zab near Aski-Kalak, Kurdistan-Iraq, morphological and molecular investigations. IOP Conference Series: Earth and Environmental Science, 761(1). https://doi.org/10.1088/1755-1315/761/1/012113
Hieu, T. K., Van, V. T. T., Thien, P. C., & Diep, D. X. (2022). Effects of different stocking densities on growth and survival rate of Dau Nhim snakehead (Channa sp.) fingerlings. AACL Bioflux, 15(3), 1124–1132. http://www.bioflux.com.ro/aacl
Hoare, C. A. (1932). On protozoal blood parasites collected in Uganda. With an account of the life cycle of the crocodile haemogregarine. Protozoal Blood Parasites, 24(2), 210–224. https://doi.org/10.1017/S0031182000020564
Jannat, B., Kanon, K. F., Sultana, N., Alam, M. S., & Alam, M. S. (2022). Morphological variations between native and Vietnam-originated striped snakeheads (Channa striata) in Bangladesh. Bangladesh Journal of Fisheries, 34(1), 41–52. https://doi.org/10.52168/bjf.2022.34.5
Jones, S. R. M., & Woo, P. T. K. (1991). Development and infectivity of Trypanosoma phaleri in leech and fish hosts. Canadian Journal of Zoology, 69(6), 1522–1529. https://doi.org/10.1139/z91-213
Kabita, F. N., Bhuiyan, M. A. I., & Jhinu, Z. N. (2020). A Checklist on the Protozoan Parasites of Freshwater Fishes of Bangladesh. Bangladesh Journal of Zoology, 48(1), 21–35. https://doi.org/10.3329/bjz.v48i1.47873
Kefil, S., Duval, L., Labat, A., Bouguerche, C., & Kechemir-Issad, N. (2025). A parasite through time: Revisiting Trypanosoma rajae Laveran and Mesnil, 1902 with new molecular and morphological insights from the blood of Rajidae in the western Mediterranean. International Journal for Parasitology: Parasites and Wildlife, 27. https://doi.org/10.1016/j.ijppaw.2025.101097
Kruse, P., Steinhagen, D., & Körting, W. (1989). Development of Trypanoplasma borreli (Mastigophora: Kinetoplastida) in the Leech Vector Piscicola geometra and Its Infectivity for the Common Carp, Cyprinus carpio. The Journal of Parasitology, 75(4), 527–530. http://www.jstor.orgURL:http://www.jstor.org/stable/3282901
Lai, D.-H., Hashimi, H., Lun, Z.-R., Ayala, F. J., & Lukeš, J. (2008). Adaptations of Trypanosoma brucei to gradual loss of kinetoplast DNA: Trypanosoma equiperdum and Trypanosoma evansi are petite mutants of T. brucei. Proceedings of the National Academy of Sciences, 105(6), 1999–2004. https://doi.org/10.1073/pnas.0711799105
Le Roux, C., Cook, C. A., Netherlands, E. C., Truter, M., & Smit, N. J. (2025). Molecular and morphological characterization of one known and three new species of fish parasitic Trypanosoma Gruby, 1972 from the south coast of South Africa. Parasitology, 152(5), 531–550. https://doi.org/10.1017/S0031182025000496
Lemos, M., Fermino, B. R., Simas-Rodrigues, C., Hoffmann, L., Silva, R., Camargo, E. P., Teixeira, M. M. G., & Souto-Padrón, T. (2015). Phylogenetic and morphological characterization of trypanosomes from Brazilian armoured catfishes and leeches reveal high species diversity, mixed infections and a new fish trypanosome species. Parasites and Vectors, 8(1). https://doi.org/10.1186/s13071-015-1193-7
Martínez, C. S., Florencio, M. L. E., & Nepomuceno, M. T. (2019). Nucleolar structure and function in trypanosomatid protozoa. Cells, 8(5), 1–22. https://doi.org/10.3390/cells8050421
McAllister, M., Phillips, N., & Belosevic, M. (2019). Trypanosoma carassii infection in goldfish (Carassius auratus L.): changes in the expression of erythropoiesis and anemia regulatory genes. Parasitology Research, 118(4), 1147–1158. https://doi.org/10.1007/s00436-019-06246-5
Mohamed Osman, H. A. E. F., Mahmoud, A. E., El-Deen, A. E. N., Zaki, M. S., & El-Metenawy, T. M. (2020). Trypanosoma infection in african sharptooth catfish clarias gariepinus with special reference to control. Pakistan Journal of Biological Sciences, 23(3), 331–338. https://doi.org/10.3923/pjbs.2020.331.338
Morioka, S., Vongvichith, B., Chanthasone, P., Phommachane, P., & Suzuki, N. (2016). Reproductive season, age estimation and growth in a striped snakehead Channa striata population in Nasaythong District, Vientiane Province, Central Laos. Aquaculture Science, 64(2), 181–191. https://www.jstage.jst.go.jp/article/aquaculturesci/64/2/64_183/_article
Muhammad, S., Chafe, U. M., Bello, M. R., Adamu, Y. A., Mohammad, A. A., Bello, A., Mera U M, Baki, A. S., & Allam L. (2017). Morphologic diversity of piscine trypanosomes in 5 wild fish species: clarias gariepinus, tilapia zillii, synodontis nigrata, bagras bayad, and mormyrus rume of North Western Nigeria. Direct Research Journal of Veterinary Medicine and Animal Science, 2(2), 20–27. http://directresearchpublisher.org/journal/drjvmas
Parveen, S., Narejo, N. T., Qadri, A. H., Dastagir, G., Chandio, M. H., Khan, P., Saddar, F., Abro, N. A., Rashid, S., & Abbas, G. (2022). Fecundity and Gonadal Periodicity of Snakehead, Channa striata from River Indus, Near Jamshoro, Sindh, Pakistan. Sarhad Journal of Agriculture, 38(5), 341–345. https://doi.org/10.17582/JOURNAL.SJA/2022/38.5.341.345
Pathak, S., Nath Sarma, N., & Mohan Goswami, M. (2023). Channa assamensis, a new species of freshwater snakehead (Teleostei: Channidae) from the Indo-Bhutan drainage of lower Brahmaputra basin of Assam, Northeast India. Species, 24(74), 1–9. https://doi.org/10.54905/disssi.v24i74.e74s1580
Sadauki, M. A., Bako, H. Y., & Badamasi, I. (2024). Detection of Blood parasites (Trypanosoma spp) in Nile Tilapia (Oreochromis niloticus Linnaeus, 1758) in Zobe Reservoir. Scientific Reports in Life Sciences, 5(4), 31–40. https://doi.org/10.5281/zenodo.14198544
Sáez, C. J., & Dean, S. (2022). Structure, function and druggability of the African trypanosome flagellum. Journal of Cellular Physiology, 237(6), 2654–2667. https://doi.org/10.1002/jcp.30778
Setyaningrum, N., Lestari, W., Krismono, & Nuryanto, A. (2022). Genetically continuous populations of Striped Snakehead (Channa striata) in the Cingcingguling River fragmented by Sempor Reservoir, Central Java, Indonesia. Biodiversitas, 23(1), 222–230. https://doi.org/10.13057/biodiv/d230128
Shahi, N., Yousuf, A. R., Rather, M. I., Ahmad, F., & Yaseen, T. (2013). First report of blood parasites in fishes from Kashmir and their effect on the haematological profile. Open Veterinary Journal, 3(2), 89–95. http://www.openveterinaryjournal.com
Sherif, A. H., Harfoush, M. A., Diab, A. S., Khalil, R. H., & Marzouk, M. S. (2024). Trypanosoma Infection in Freshwater Fish and Its Corellation with Water Quality. In Egyptian Journal of Aquatic Biology & Fisheries (Vol. 28, Number 5, pp. 981–996). www.ejabf.journals.ekb.eg
Smit, N. J., Joubert, A., Lawton, S. P., Hayes, P. M., & Cook, C. A. (2020). Morphological and molecular characterization of an African freshwater fish trypanosome, including its development in a leech vector. International Journal for Parasitology, 50(10–11), 921–929. https://doi.org/10.1016/j.ijpara.2020.06.004
Sudasinghe, H., Ranasinghe, R. H. T., Pethiyagoda, R., Meegaskumbura, M., & Britz, R. (2021). Genetic diversity and morphological stasis in the Ceylon Snakehead, Channa orientalis (Teleostei: Channidae). Ichthyological Research, 68(1), 67–80. https://doi.org/10.1007/s10228-020-00768-0
Taslim, N. A., Fitriana, N., Suprapti, N. L. E., Marsella, C. P., Bukhari, A., Rasyid, H., Aminuddin, A., As’ad, S., Aman, A. M., & Madjid, M. (2022). Effects of Channa striata Extract on Albumin Serum Neutrophil-to-Lymphocyte Ratio in Hyperglycemic Rats Wound Injury: A Randomized Control Study. Open Access Macedonian Journal of Medical Sciences, 10, 450–455. https://doi.org/10.3889/oamjms.2022.8179
Virgilio, L. R., Teixeira, G. O., Almeida, L. S., Melo, H. P. S., Júnior, S. L. P., Meneguetti, D. U. O., Camargo, L. M. A., & Takemoto, R. M. (2022). Infection with Trypanosoma spp. in Platydoras armatulus (Siluriformes, Doradidae), in southwestern Amazon, Brazil. Journal of Parasitic Diseases, 46(3), 607–612. https://doi.org/10.1007/s12639-022-01485-3
Wanja, D. W., Mbuthia, P. G., Waruiru, R. M., Mwadime, J. M., Bebora, L. C., Nyaga, P. N., & Ngowi, H. A. (2020). Fish Husbandry Practices and Water Quality in Central Kenya: Potential Risk Factors for Fish Mortality and Infectious Diseases. Veterinary Medicine International, 2020. https://doi.org/10.1155/2020/6839354
Xu, Z., Yang, C., Gofarov, M. Y., Eliseeva, T. A., Kondakov, A. V., Yuan, H., Bolotov, I. N., & Yang, D. (2021). A new freshwater leech species from Asian Swamp Eel stocks in China. Parasitology Research, 120(8), 2769–2778. https://doi.org/10.1007/s00436-021-07228-2
Yulintine, Y., Hendri, B., Linda, W., & Edison, H. (2017). Snakehead Fish (Channa Striata): Semi-Induced Breeding and Larval Growth. Indian Journal of Science and Technology, 10(11), 1–8. https://indjst.org/articles/snakehead-fish-channa-striata-semi-induced-breeding-and-larval-growth
